Table I. S. cerevisiae strains used in this work.

Strain	Genotype	Reference
RH2817	MATα, ura3-52, trp1::hisG	40
RH3263	MATα, ura3-52, trp1::hisG, leu2::hisG, Δasc1::LEU2	40
RH3493	MATα, ura3-52, trp1::hisG, Δarg4::loxP, Δlys1::loxP	23
RH3494	MATα, ura3-52, trp1::hisG, leu2::hisG, Δasc1::LEU2, Δarg4::loxP, Δlys1::loxP	This work
BY4741	MATa, ura3Δ0, leu2Δ0, his3Δ1, met15Δ0	Euroscarf
Y03444	MATa, ura3Δ0, leu2Δ0, his3Δ1, met15Δ0, Δsro9::kanMX	Euroscarf
Y06556	MATa, ura3Δ0, leu2Δ0, his3Δ1, met15Δ0, Δasc1::kanMX	Euroscarf
RH3694 RH3695	MATa, ura3Δ0, leu2Δ0, his3Δ1, met15Δ0, Δsro9::kanMX, Δasc1::URA3	This work
RH3686	MATa, ura3Δ0, leu2Δ0, his3Δ1, met15Δ0, SRO9::GFP::HIS3MX	Invitrogen, 75
RH3687	MATa, ura3Δ0, leu2Δ0, his3Δ1, met15Δ0, UBP3::GFP::HIS3MX	Invitrogen, 75
RH3696 RH3697	MATa, ura3Δ0, leu2Δ0, his3Δ1, met15Δ0, UBP3::GFP::HIS3MX, Δasc1::URA3	This work
W303-1a	W303, MATa, ura3, leu2-3 112, his3-11 15, trp1-1, ade2-1, can1-100	34
JSY1194	W303, MATa, ura3, leu2-3 112, his3-11 15, trp1-1, ade2-1, can1-100, eGFP-DEF1	34
RH3698	W303, MATa, ura3, leu2-3 112, his3-11 15, trp1-1, ade2-1, can1-100, Δasc1::URA3	This work
JSY1198	W303, MATa, ura3, leu2-3 112, his3-11 15, trp1-1, ade2-1, can1-100, 9xMyc-2xTEV-6xHis-DEF1	34
RH3699	W303, MATa, ura3, leu2-3 112, his3-11 15, trp1-1, ade2-1, can1-100, 9xMyc-2xTEV-6xHis-DEF1, Δasc1::URA3	This work