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. 2017 Oct 10;16(12):2243–2253. doi: 10.1074/mcp.RA117.000296

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© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

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Fig. 3. — BfrB binds to the N terminus of RPS3. A, Diagram of RPS3 and EGFP-tagged truncation mutants. KH, K homology. B, HEK293T cells transfected with constructs expressing FLAG-tagged BfrB and full-length or truncated EGFP-tagged RPS3 proteins, lysed after 48 h, and immunoblotted for EGFP-tagged RPS3 proteins after immunoprecipitation with FLAG antibody for BfrB in lysates. Experiments were carried out in triplicate. C, Detailed view of the interaction between the RPS3 (pale green) and BfrB (pink); Amino acids 164–181 of the BfrB were showed in rose red, and amino acids 1–42 of the RPS3 were displayed in green.