Overexpression of Mtb BfrB in Msm inhibits the expression of TNF-α and IL-1β and promotes the survival of mycobacteria in macrophages.
A, B, Quantitative PCR analysis of TNF-α mRNA (A) and IL1β mRNA (B) in PMA-differentiated THP-1 macrophages infected for 0–24 h with wild-type Msm or Msm with bfrB-overexpressing. Experiments were carried out in triplicate. C, Survival of Msm in PMA-differentiated THP-1 macrophages infected for 0–48 h with wild-type Msm or Msm with bfrB-overexpressing 0.01<*p < 0.05, 0.001<**p < 0.01, ***p < 0.001 (two-tailed unpaired t test). Data are representative of one experiment with three independent biological replicates.