Fig. 5.

Verification of MIS18BP1 and KIF23 as co-modified targets upon inhibition of the proteasome. Parental U2OS cells, U2OS cells expressing His₁₀-ubiquitin and U2OS cells expressing His₁₀-ubiquitin and FLAG-SUMO2 were treated with DMSO or MG132 to inhibit the proteasome. Samples were taken before the His₁₀-pulldown (input), after the His₁₀-pulldown (PD) and after the FLAG-IP (PD+IP), and analyzed by immunoblotting with antibodies against MIS18BP1, KIF23, polyHistidine and FLAG. An equal percentage of the sample was loaded for the PD and PD+IP samples to enable comparison. The asterisk represents an a-specific band.