Table 1.
Primer name | Primer sequence (5′ to 3′)a | Purpose |
---|---|---|
CR1-vigs-F | GGATCCGGATTATCAACAGGCTGATTC | Target fragment clone |
CR1-vigs-R | CTCGAGGCAAGAGAGGCAAGGCATTT | Target fragment clone |
CR1-F | GCTGCACTCAGGTTCAGAGG | Target gene in qRT-PCR |
CR1-R | GCAAGAGAGGCAAGGCATTT | Target gene in qRT-PCR |
CR2-F | CAAACCACTATACCCGCCGT | Target gene in qRT-PCR |
CR2-R | GAAGAATCCGTTGCGTCAGC | Target gene in qRT-PCR |
CR3-F | GCCGTCGAAAAGAAAAACCCA | Target gene in qRT-PCR |
CR3-R | GCGGTGTCAAATGTTCCCAA | Target gene in qRT-PCR |
STR-F | GCCTTCACCTTCGATTCAACTG | Target gene in qRT-PCR |
STR-R | GTGGCTAGTTGTGTGGCATACC | Target gene in qRT-PCR |
G10H-F | GCTCACAATCAATTCAAATTCTCCGT | Target gene in qRT-PCR |
G10H-R | CAACCGCTTCTCCGCTCTGGCTATTT | Target gene in qRT-PCR |
TDC-F | GGTCGAGGATGACGTGGCGGCCGG | Target gene in qRT-PCR |
TDC-R | ACTCAGACTCAGTGAGTCAACTCGTT | Target gene in qRT-PCR |
PRX1-F | GCGATTCATCAGTGCTGCTGGTGGGA | Target gene in qRT-PCR |
PRX1-R | GTGGAAGGTTTGCTATTGTGTCTGCC | Target gene in qRT-PCR |
SLS-F | CTTCACTCTTGAGAAACTAAAGTCAA | Target gene in qRT-PCR |
SLS-R | AGTCAATTGTGAGATCCATGAGTT | Target gene in qRT-PCR |
DAT-F | CTTCTTCTCATCACGTACCAACTC | Target gene in qRT-PCR |
DAT-R | ATACCAAACTCAACGGCCTTAG | Target gene in qRT-PCR |
SGD-F | CATTGGTGAACCGTGCTATG | Target gene in qRT-PCR |
SGD-R | AGATTGTAGAGTCCAGATGGAACA | Target gene in qRT-PCR |
RPS9-F | GCGTTTGGATGCTGAGTTGAAG | Endogenous reference gene |
RPS9-R | GGCGCTCAAGGAAGTTCTCTAC | Endogenous reference gene |
aThe underlined letters indicate the restriction enzyme sites.