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. 2017 Nov 20;114(49):E10532–E10539. doi: 10.1073/pnas.1711534114

Fig. 2.

Fig. 2.

EMT programs induce Hh pathway activation. (A and B) Relative levels of the indicated gene transcripts were determined by real-time qPCR analysis (n = 3, mean ± SEM) of FACS-sorted luminal cells, MaSC-enriched basal cells, and stromal cells (A) and HMLE or HMLER cells in the E-like [CTL, sh (short hairpin)CTL] versus M-like (SNAIL, shEcad) states (B). *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. Representative results from three independent experiments are shown. (C) Colocalization of GLI2 and GLI3 with the cilium marker Arl13b in MaSC-enriched basal cells and HMLER cells in the M-like state (shEcad). (Scale bar, 5 μm.) Insets: 2× magnification.