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. 2017 Nov 20;114(49):E10532–E10539. doi: 10.1073/pnas.1711534114

Fig. 3.

Fig. 3.

Hh pathway induction relies on primary ciliogenesis. (A) KIF3A and IFT20 knockouts were validated by Western blot of extracts from the indicated cells. (B and C) The impact on morphology and ciliogenesis was assessed as described in the legend of Fig. 1. (Scale bars: brightfield, 50 μm; immunofluorescence, 15 μm.) (D) Relative GLI1 and GLI2 RNA levels (n = 3, mean ± SEM) in indicated control (WT) and mutant (MT) cells were analyzed by real-time qPCR. Representative results from three independent experiments are shown. (E and F) The impact of ciliobrevin A on ciliogenesis and GLI-TF RNA levels in HMLER cells in the M-like state (shEcad) were analyzed as described above. **P ≤ 0.01, ***P ≤ 0.001.