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. 2017 Nov 14;114(49):E10540–E10549. doi: 10.1073/pnas.1711538114

Table 4.

Single injection of multiple sgRNAs into the embryos of AAEL010097-Cas9 line results in highly efficient rates of multigene disruption

Injected sgRNAs	Injected embryos	G₀ adult survivors (%)	G₀ mosaic (%)	Single mutant G₀s (%); G₁s^* (%)			Double-mutant G₀s (%); G₁s^† (%)			Triple-mutant G₀s (%); G₁s^‡ (%)
Injected sgRNAs	Injected embryos	G₀ adult survivors (%)	G₀ mosaic (%)	W	Y	E	W/Y	W/E	Y/E	W/Y/E
W1-sgRNA/Y-sgRNA	200	122 (61)	110 (90)	4 (4); 170 (15)	7 (6); 238 (21)	N/A, N/A	99 (90); 488 (43)	N/A; N/A	N/A; N/A	N/A; N/A
W1-sgRNA/E-sgRNA	200	113 (56)	98 (87)	7 (7); 141 (17)	N/A; N/A	5 (5); 224 (27)	N/A; N/A	86 (88); 390 (47)	N/A; N/A	N/A; N/A
Y-sgRNA/E-sgRNA	200	102 (51)	95 (93)	N/A; N/A	3 (3); 343(25)	5 (5); 261 (15)	N/A; N/A	N/A; N/A	87 (92); 536 (39)	N/A; N/A
W1-sgRNA/Y-sgRNA/E-sgRNA	200	86 (43)	81 (94)	3 (4); 85 (11)	3 (4); 54(7)	1 (1); 23 (3)	5 (6); 131 (17)	9 (11); 101 (13)	6 (7); 162 (21)	54 (67); 85 (11)

N/A, not applicable.

^*

Single-mutant phenotype, W: white eye; Y: yellow body; E: dark body.

^{^†}

Double-mutant phenotypes, W/Y: white eye and yellow body, W/E: white eye and dark body; Y/E yellow body and dark body.

^{^‡}

Triple-mutant phenotypes, W/Y/E: white eye, yellow body, and dark body.