Table 5.

Transgenic source of Cas9 results in increased rates of HDR with dsDNA donors

Ae. aegypti strain	Injected component*	Injected embryos	Adult G0 survivors (%)	HDR event
				No. group founders/total group (%)	No. HDR G1/total G1 (%)
Liverpool (wild-type)	W1-sgRNA/Cas9/white-donor	600	251(41.83)	1/20 (5)	9/5,371 (0.15)
Liverpool (wild-type)	Kh-sgRNA/Cas9/kh-donor	600	273 (45.50)	1/20 (5)	7/4,773 (0.14)
AAEL010097-Cas9	W1-sgRNA/white-donor	600	308 (51.33)	4/20 (20)	118/4,993 (2.36)
AAEL010097-Cas9	Kh-sgRNA/kh-donor	600	298 (49.67)	5/20 (25)	149/6,017 (2.48)

^*The concentration of sgRNA is 100 ng/μL; Cas9 protein 300 ng/μL. white-donor and kh-donor plasmids 100 ng/μL.