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. 2017 Dec 3;23:5744–5751. doi: 10.12659/MSM.905336

Figure 1.

Figure 1

miR-1273g-3p was highly expressed in STZ-induced DR RPE cells. (A) Differential expression analysis was performed using Cuffdiff. RPE cells were induced by clusterin and an mRNA library was constructed. Protein-coding transcripts and lncRNAs with fold change (FC) >1.5 or FC <0.67 and P value <0.05 were considered to be differentially expressed in RPM cells treated by clusterin; miRNAs with FC >2 or FC <0.5 were considered aberrantly expressed. (B) miR-1273g-3p was highly expressed in STZ-induced DR RPE cells. Total RNA was isolated from NC, DM, DM + inhibitor, and DM + mimic groups for qRT-PCR. NC, RPE cells from normal SD rats; DM, RPE cells from STZ-induced DM rats; DM + inhibitor, RPE cells from STZ-induced DM rats pretreated with miR-1273g-3p inhibitor; DM + mimic, RPE cells from STZ-induced DM rats pretreated with miR-1273g-3p mimic. U6 was used as the internal control to miR-1273g-3p. The mRNA level of C5AR2 was normalized to β-actin. * P<0.05, ** P<0.01.