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. Author manuscript; available in PMC: 2017 Dec 11.
Published in final edited form as: Cell Rep. 2014 Sep 4;8(5):1571–1582. doi: 10.1016/j.celrep.2014.08.003

Figure 5. A specific TASK-3 antagonist shifts the temperature threshold of TRPM8 neurons.

Figure 5

(A) Time course of current block caused by Compound 23 (C23) and pH 6.0 on whole cell-currents at 0 mV in HEK293 cells transfected with mouse TASK-3. Currents were activated by 1-s duration voltage ramps from −120 to +60 mV at 33 °C. (B) Current-voltage relationship of whole cell-currents and the effects of C23 (200 nM and 1 μM) and pH 6.0. The corresponding current values at 0 mV are marked with large filled circles in A. (C) Summary histogram of the effects of C23 and pH 6.0 on whole cell-currents at 0 mV. C23 blocks TASK-3-currents (84.0 ± 1.5% inhibition at 200 nM; n = 5) and (93.4 ± 1.6% inhibition at 1 μM; n = 4). pH 6.0 produced a 97.1 ± 0.3% inhibition (n = 5). Each response to C23 or pH 6.0 was normalized and compared to the preceding response in control solution (*** for p<0.001; paired t-test). (D) Time course of Fura-2 calcium response in a EYFP(+) neuron challenged with three consecutive cooling pulses, before, during and after exposure to Compound 23 (1 μM). (E) Effect of Compound 23 (1 μM) on the cold-evoked threshold of 13 EYFP(+) neurons. The neuron depicted in D is represented by green triangles. (F) Mean ± sem effect of Compound 23 on thermal threshold of EYFP(+) neurons (*** for p<0.001, paired t-test).