Table 1.
PCR primers used for the site-mutation of CYP3A4
| Variants | cDNA changes | Reverse primer (5′→3′) | Forward primer (5′→3′) |
|---|---|---|---|
| CYP3A4*2 (S222P) | 664T>C | CTGTTATTGGGAGAAAGAA | TTCTTTCTCCCAATAACAG |
| CYP3A4*3 (M445T) | 1334T>C | GCAAACCTCGTGCCAATGC | GCATTGGCACGAGGTTTGC |
| CYP3A4*4 (I118V) | 352A>G | CTATAGAGACGGCACTTTT | AAAAGTGCC |
| CYP3A4*5 (P218R) | 653C>G | AGAAAGAATCGATCCAAA | TTTGGATCGATTCTTTCT |
| CYP3A4*9 (V170I) | 508G>A | TCAAGGTGATAGGCTTGCC | GGCAAGCCTATCACCTTGA |
| CYP3A4*10 (D174H) | 520G>C | CAAAGACGTGTTTCAAGGT | ACCTTGAAACACGTCTTTG |
| CYP3A4*11 (T363M) | 1088C>T | AATCTGAGCATTTCATTCA | TGAATGAAATGCTCAGATT |
| CYP3A4*14 (L15P) | 44T>C | ACCAGTCGACATGGCTCTCATCCCAGAC | TTGGCCATGGAAACCTGGCTTCTCCCGGCTG |
| CYP3A4*15 (R162Q) | 485G>A | TCTGCTTCCTGCCTCAGAT | ATCTGAGGCAGGAAGCAGA |
| CYP3A4*16 (T185S) | 554C>G | GATGTGCTACTGATCACAT | ATGTGATCAGTAGCACATC |
| CYP3A4*17 (F189S) | 566T>C | TTCACTCCAGATGATGTGC | GCACATCATCTGGAGTGAA |
| CYP3A4*18 (L293P) | 878T>C | ACGAGCTCCGGATCGGACA | TGTCCGATCCGGAGCTCGT |
| CYP3A4*19 (P467S) | 1399C>T | CTTTACAAGATTTGAAGGA | TCCTTCAAATCTTGTAAAG |
| CYP3A4*23 (R162W) | 484C>T | CTGCTTCCCACCTCAGATT | AATCTGAGGTGGGAAGCAG |
| CYP3A4*24 (Q200H) | 600A>T | AAAGGGGTCATGTGGATTG | CAATCCACATGACCCCTTT |
| CYP3A4*28 (L22V) | 64C>G | ATAGATAGACGAGCACCAG | CTGGTGCTCGTCTATCTAT |
| CYP3A4*29 (F113I) | 337T>A | TTTTCATAATTCCCACTGG | CCAGTGGGAATTATGAAAA |
| CYP3A4*30 (R130STOP) | 388C>T | GCAATGATCATAATCTCTT | AAGAGATTATGATCATTGC |
| CYP3A4*31 (H324Q) | 972C>A | GACATCAGGTTGAGTGGCC | GGCCACTCAACCTGATGTC |
| CYP3A4*32 (I335T) | 1004T>C | ACTGCATCAGTTTCCTCCT | AGGAGGAAACTGATGCAGT |
| CYP3A4*33 (A370S) | 1108G>T | GTCTCATAGAAATTGGGAA | TTCCCAATTTCTATGAGAC |
| CYP3A4*34 (I427V) | 1279A>G | AAGGATCTACGTTGTCCTT | AAGGACAACGTAGATCCTT |
Note: The positions where the nucleotides are exchanged are indicated with underlined bold characters.