Figure 9.

GPR37L1 is neuroprotective during chemical ischemia in vitro. Hippocampal slices from P14‐P16 Gpr37l1 ^+/+ and Gpr37l1 ^–/– mice were incubated for 30 min in control or ischemic solution containing propidium iodide (PI), followed by 40 min in nonischemic solution, and subsequently labelled for NeuN. (a) NeuN cells labelled for PI were visible after ischemia in the pyramidal layer. (b) Example of a Gpr37l1 ^+/+ pyramidal neuron labelled for NeuN (c) and PI (d) after ischemia. (e) Percentage of dead cells for control or ischemia in Gpr37l1 ^+/+ and Gpr37l1 ^–/– littermates (n = 6 experiments). (f) Percentage of dead cells for control or ischemia, alone or with prosaptide (pro) included in the ischemic solution, in Gpr37l1 ^+/+ and Gpr37l1 ^–/– littermates (n = 4 experiments). All p values are corrected for multiple comparisons