Table 1.
Previous in vitro studies of myoblast growth and differentiation using silk protein matrices
| Silk matrix type | Cell type | Experiment | Outcome | Reference |
|---|---|---|---|---|
| Fibroin*–polyurethane blended films | C2C12 mouse myoblasts | Compatibility and differentiation | Silk fibroin improved the cell compatibility of polyurethane films | Park et al. (2013b) |
| Fibroin* immobilized on polyurethane membranes | Cells from skeletal muscle biopsy (primary hypopharynx myoblasts) | Compatibility and differentiation | Cell proliferation, alignment and myofibre differentiation achieved in aligned microchannels | Shen et al. (2013) |
| Fibroin*–tropoelastin films | C2C12 cells and human bone marrow stem cells (hMSCs) | Cell attachment, proliferation and myogenic lineage differentiation | Roughness & stiffness favouring proliferation and differentiation of C2C12 cells or hMSCs determined | Hu et al. (2011) |
| Recombinant spider silk protein eADF4(C16) film | C2C12 mouse myoblasts | Cell adhesion and orientation | Myoblasts adhered to and proliferated best on structured, not unstructured films | Bauer et al. (2013) |
| Sericin* supplemented serum‐free medium | C2C12 mouse myoblasts | Myoblast differentiation | Myotubes formed with sarcomeres | Fujita et al., 2010 (Fujita et al., 2010) |
*
Silk protein obtained from Bombyx mori, a mulberry silkworm species.