Table 2.

Primer Information

Gene	Sequence (5′–3′)	NCBI ref.	Primer Bank ID	TM (°C)	Length (bp)
SDHA	F: TGG CAT TTC TAC GAC ACC GTG R: GCC TGC TCC GTC ATG TAG TG	NM_004168	156416002c3	54, 56	77
ACTAB	F: CATGTACGTTGCTATCCAGGC R: CTCCTTAATGTCACGCACGAT	NM_001101	4501885a1	61, 60	250
TBP	F: CCC GAA ACG CCG AAT R: ATA ATC C AAT CAG TGC CGT GGT TCG TG	NM_003194	285026518c2	55, 54	80
MYOD1	F: CGG CAT GAT GGA CTA CAG CG R: CAG GCA GTC TAG GCT CGA C	NM_002478	77695919c2	56, 55	133
MYF5	F: CTG CCA GTT CTC ACC TTC TGA R: AAC TCG TCC CCA AAT TCA CCC	NM_005593	156104905c1	54, 54	78
ACTA1	F: GGC ATT CAC GAG ACC ACC TAC R: CGA CAT GAC GTT GTT GGC ATA C	NM_002478	47078293c1	56, 55	84
MYH1	F: GGG AGA CCT AAA ATT GGC TCA A R: TTG CAG ACC GCT CAT TTC AAA	NM_005963	115527081c2	53, 50	106
MYH7	F: TGG ATG TGA GTG AAC TTG GGG R: GCA CCC AGA CTC GCT TCT T	NM_020884	291045202c2	54, 53	108

F, forward; R, reverse.