Figure 3.

Confocal images showing the association between cells NGF+ blood vessels and nerve fibers expressing p75, TrkA, CGRP, and TH. In these confocal images (all of which were obtained in the bone marrow), nerve fibers expressing p75 (A), Trk A (B), CGRP (C and E) and TH (D and E) appear to be closely associated with NGF+ blood vessels (yellow in A–D) that vascularize the bone marrow. Also note (arrowheads) that there are a few single NGF+ cells (A–D) that are spherical and have a very different morphology and do not appear to be closely associated with the NGF+ blood vessels. Confocal images shown in (A–D) are from 60 µm sections, whereas (E) is a merged image showing the relationship between a NGF+ blood vessel and CGRP+ sensory nerve fibers and TH+ post-ganglionic sympathetic nerve fibers with a total z-stack = 120 µm. Line bar = 60 µm.