Figure 6.
IL-27 improves rapamycin-induced autophagy of UECC. (A) The transcription level of BECN, MAP1LC3B, MTOR, and SQSTM1 in Ishikawa cells treated as described in Figure 4A was analyzed by RT-PCR. (B, C) TEM was used to analyze the autophagy levels of cancer lesions from Ishikawa (NC or IL-27over)-xenografted nude mice (n = 8 mice/group), which were peritoneally injected with or without rapamycin and (D, E) transferred with peripheral human NK cells. The autophagy grade was based on the average number of autophagic vacuoles and autolysosome/per cell visible by TEM. Data are expressed as mean ± SEM. *P < .05, **P < .01, ***P < .001, and ****P < .0001.