Fig 1. Increased severity and mortality of M. marinum pulmonary infection in Ncf1^-/- mice.

Ncf1^-/- (loss of function mutation in p47phox) and WT controls were intra-tracheal injected with M. marinum (3 x 10⁷ CFU). Survival (A) and changes in body weight (B) were monitored over the 28 days period following M. marinum infection in WT (n = 20) and Ncf1^-/- mice (n = 23). The number of viable mycobacteria (C) was determined at 7 days and 14 days after M. marinum infection. Data are shown as a mean log of CFU per paired-lung (5 mice per group). The high correlation between changes in body weight and a number of viable mycobacteria in lungs was demonstrated in (D). Data represented mean ± sd. The experiments were analyzed with Log-rank test (A), Kruskal-Wallis test (C), and Spearman’s rank correlation analysis (D) *p < 0.05; **p < 0.005. These experiments were repeated twice with similar results.