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. 2017 Dec 11;12(12):e0189453. doi: 10.1371/journal.pone.0189453

Fig 7. IL-1β depletion and IL-1 receptor antagonist treatment alleviated the neutrophilic inflammation.

Fig 7

The characteristic pulmonary histopathological images (A) of M. marinum-infected Ncf1-/- mice and WT mice on day-5 with and without IL-1β depletion by a monoclonal antibody directed against IL-1β (150μg on day-1). The immunofluorescent stain of neutrophil elastase (NE) (B) also showed an apparently decrease of NE-positive cells after the depletion of IL-1β. The representative histological images (C) of WT and Ncf1-/-mice treated with and without IL-1 receptor antagonist (Anakinra, 100mg/kg/day for 5days). Percentages of infiltration area (D) were quantified by using Image J. The experiments were analyzed with Kruskal-Wallis test and repeated with similar results. *p < 0.05; **p < 0.005.