Fig 7. CT51 is a reversible intracellular iron sensor.

The graph shows the fluorescence recorded in SH-SY5Y cells incubated with CT51 (20 min, 10 μM) and detected in a fluorescence plate reader (λ Ex. 340 nm; λ Em. 460 nm). (a) Basal CT51 fluorescence in cells cultured for 24 h with 20 μM Fe. (b) CT51 fluorescence recorded after addition of the Fe²⁺ chelator bipy (20 μM). (c) CT51 fluorescence recorded after addition of 80 μM FAS. Values represent Mean ± SEM; n = 3.