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. 2017 Dec 11;12(12):e0189379. doi: 10.1371/journal.pone.0189379

Fig 4. T22 reporter assay measuring p53 transactivation.

Fig 4

A. Cells were treated with increasing concentrations (5, 10, 20 μM) of indicated peptide ligands and p53 activity determined by measuring β galactosidase levels. The small molecule Mdm2 inhibitor Nutlin (10 μM) was used as positive control. Activity is expressed as fold increase over cells treated with DMSO vehicle only. Values represent average ± SD (n = 2). * p < 0.001 compared to DMSO control. B. Lactate dehydrogenase release assay indicating no significant membrane disruption observed for increasing concentrations (2, 10, 20 μM) of indicated ligands compared to vehicle (DMSO) and positive maximum lysis control (MLC). Values represent average ± SD (n = 3).