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. 2017 Dec 11;12(12):e0189167. doi: 10.1371/journal.pone.0189167

Fig 1. IgG deposition in the transgenic ear tissue increases with phenotypic stage and age.

Fig 1

Protein (50μg/track) was extracted from L2LMP1 transgenic ears (stages 1 to 5, upper blot as indicated) and control (NSC lower blot) of mice of increasing age and western blotted (age in days, as indicated above or below (respectively)). The blots were probed with antibodies to actin (42kD) and GAPDH (37kD), using anti-mouse IgG as secondary, also detecting tissue immunoglobulin-G: IgH and IgL, as indicated. Protein size markers in kD are indicated to the left of each panel. Below: the normalised values (IgH/actin) are shown graphically for transgenic (L2LMP1) and NSC samples, plotted against age.