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. 2016 Nov 22;13(2):345–358. doi: 10.1080/15548627.2016.1256521

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Figure 3. — Transiently expressed ATG16L1⁺ puncta were negative for the ATG12–ATG5 conjugate and ULK1. (A) A549 cells were transduced with Ad-mCherry-ATG16L1 vector (MOI 2) for 16 h. Cells were then starved with EBSS for 2 h before they were fixed and probed with ATG12 antibody. (B) A549 cells stably expressing GFP-ATG16L1 were starved with EBSS for 2 h before they were fixed and probed with ATG12 antibody. (C) A549 cells were transduced with Ad-mCherry-ATG16L1 vector (MOI 2) for 16 h. Cells were then starved with EBSS for 2 h before they were fixed and probed with ULK1 antibody. (D) A549 cells stably expressing GFP-ATG16L1 were starved with EBSS for 2 h before they were fixed and probed with ATG12 antibody. Arrows point to starvation-induced GFP-ATG16L1 puncta positive for ATG12 or ULK1. Scale bar: 25 micron.