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. 2017 Dec 4;6:e33323. doi: 10.7554/eLife.33323

Figure 4. Jag1 and cLmx1b are expressed in complementary domains and Notch inhibits cLmx1b expression in the chick otocyst.

Figure 4.

(A–B’’) Fluorescent in situ hybridization for cLmx1b (magenta) and immunostaining for Jag1 (green) in the E3 chick otocyst. (A, A’’) Jag1 is expressed in a complementary manner to cLmx1b. (B–B’’) High magnification view of the anterior region of the otocyst shown in (A). (C–D) Whole-mount in situ hybridization for cLmx1b 24 hr after in ovo electroporation with plasmids encoding either NICD (C) or a control red fluorescent protein (D) used at the same concentration. Note the reduction in cLmx1b expression in the NICD-transfected sample (C, right) in comparison to the untransfected contralateral otocyst (C, left) or to the otocyst transfected with a control plasmid (D, right).