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. 2017 Dec 4;6:e33323. doi: 10.7554/eLife.33323

Figure 5. Over-expression of cLmx1b down-regulates expression of Jag1.

Figure 5.

(A–A’’) Whole mount preparation of an E4.5 chick utricle after electroporation with the pT2K-Lmx1-eGFP Tol2 construct and immunostaining for Jag1. Note the irregular contours of the utricular macula and the reduced expression of Jag1 in transfected cells (asterisk). (B–B’’) Whole mount view of an Lmx1-eGFP transfected chick inner ear at E7. Jag1 expression is reduced or completely absent in cLmx1b-overexpressing cells, resulting in abnormal positioning of the boundaries of the utricle (ut) and saccule (sac) (arrows in B’–B’’,) see also Video 1 for a 3D animation of a high magnification view of this region) and a large truncation (dotted line) of the basilar papilla (bp). (C) Surface view of an Lmx1-eGFP transfected E7 basilar papilla with several groups of cLmx1b-overexpressing cells (arrowheads). At higher magnification (D–D’’), note the rounded appearance of the clusters of transfected cells (D’) and the absence of Jag1 expression in those cells (D’’). (E–E’’) Surface view of an eGFP (control) transfected E7 basilar papilla. There is no disruption in the expression pattern of Jag1 or the positioning of its lateral borders.