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. 2017 Dec 4;6:e33323. doi: 10.7554/eLife.33323

Figure 6. Overexpression of cLmx1b down-regulates Sox2 expression in a context-dependent manner.

Figure 6.

(A–E’) Transverse views of the developing chick inner ear following electroporation with pT2K-Lmx1-eGFP and immunostaining for Sox2 (magenta) and HCA (cyan). (A) An E4.5 sample with two vestibular sensory patches containing transfected cells. At high magnification (B–B’’), note that the levels of Sox2 are unchanged in cLmx1b-overexpressing cells (arrowheads) compared to neighbouring untransfected cells (B–B’). (C) Low magnification view of an E7 sample, with the utricle (ut), saccule (sac) and anterior crista (cr) visible. (D–D’) High magnification view of the utricle, containing cLmx1b-overexpressing cells that retain Sox2 expression (arrows), although at a reduced level compared to neighbouring untransfected cells. (E–E’) A cluster of transfected cells is abutting the anterior crista, and show a complete absence of Sox2 expression. Note also the apical constriction of the transfected cells (arrowheads).