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. 2017 Oct 9;8(59):100021–100033. doi: 10.18632/oncotarget.21754

Figure 2. Glipizide induces autophagy and sensitized apoptosis mediated by TRAIL.

Figure 2

A549 cells were pre-incubated with glipizide at varying doses (0, 25, 50, and 100 μM) for 12 h. (A and B) Western blot for DR-4, DR-5, LC3-II, and p62 proteins was analyzed from A549 cells; (C) Cells were immunostained with p62 antibody (red) and observed in fluorescent view; (D) TEM shows the ultrastructure of cells treated with glipizide for 12 h. Arrows indicate autophagosomes, together with residual digested material and empty vacuoles; (E) Western blot for Ac-cas3 and Ac-cas8 expression levels was conducted with A549 cells. Cells were pre-incubated with glipizide for 12 h and exposed to TRAIL protein for an additional 1 h. β-actin was used as the loading control. Gli: Glipizide; TRAIL: Tumor necrosis factor (TNF)-related apoptosis-inducing ligand; Ac-cas3: Activated caspase 3; Ac-cas8: Activated caspase 8.