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. 2017 Oct 9;8(59):100021–100033. doi: 10.18632/oncotarget.21754

Figure 3. Glipizide enhanced TRAIL-induced apoptosis is blocked by inhibition of autophagy.

Figure 3

Cells were pre-incubated with the indicated glipizide doses for 12 h and exposed to TRAIL protein for an additional 2h. Additional cells were also pre-incubated with autophagy inhibitor chloroquine for 1 h followed by glipizide treatment. (A) Cell morphology photographed using light microscope (×100); (B) Cell viability was measured with crystal violet assay; (C) Bar graph indicating average density of crystal violet; (D) Cell viability was measured with trypan blue dye exclusion assays. ** p<0.01, *** p < 0.001: represent significant differences between control and each treatment group; Gli: Glipizide; TRAIL: Tumor necrosis factor (TNF)-related apoptosis-inducing ligand; CQ: Chloroquine.