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. 2017 Oct 9;8(59):100021–100033. doi: 10.18632/oncotarget.21754

Figure 4. Autophagy inhibitor blocks TRAIL mediated apoptosis by glipizide via activating autophagy flux.

Figure 4

A549 cells were pre-incubated with chloroquine for 1h followed by indicated glipizide doses for 12 h. (A and B) Western blot for DR-4, DR-5, LC3-II, and p62 proteins was analyzed from A549 cells; (C) Cells were immunostained with p62 antibody (red) and observed in fluorescent view; (D) Western blot for Ac-cas3 and Ac-cas8 expression levels was conducted with A549 cells. Cells were pre-incubated with the indicated glipizide concentrations for 12 h and exposed to TRAIL protein for an additional 1h. Additional cells were pre-incubated with autophagy inhibitor chloroquine for 1 h, followed by glipizide treatment. β-actin was used as the loading control. Gli: Glipizide; Tumor necrosis factor (TNF)-related apoptosis-inducing ligand; Ac-cas3: Activated caspase 3; Ac-cas8: Activated caspase 8; CQ: Chloroquine.