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. 2017 Oct 9;8(59):100021–100033. doi: 10.18632/oncotarget.21754

Figure 5. Glipizide enhanced TRAIL-induced apoptosis is blocked by genetic inhibition of autophagy.

Figure 5

A549 cells were pre-incubated with ATG5 siRNA or negative control siRNA for 24 h and then exposed to indicated glipizide doses for 12 h with or without TRAIL protein for an additional 2 h. (A) Cell morphology photographed using light microscope (×100); (B) Cell viability was measured with crystal violet assay; (C) Bar graph indicating average density of crystal violet; (D) Cell viability was measured with trypan blue dye exclusion assays. ** p<0.01, *** p < 0.001: represent significant differences between control and each treatment group. Gli: Glipizide; TRAIL: Tumor necrosis factor (TNF)-related apoptosis-inducing ligand; siATG5: ATG5 small interfering RNA; NC: Negative control.