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. 2017 Oct 9;8(59):100021–100033. doi: 10.18632/oncotarget.21754

Figure 6. Genetic autophagy inhibitor blocks TRAIL-induced apoptosis by glipizide via activation of autophagic flux.

Figure 6

A549 cells were pre-incubated with ATG5siRNA or negative control siRNA for 24 h, and then exposed to indicated glipizide doses for 12 h. (A and B) Western blot for DR-4, DR-5, LC3-II, p62 and ATG5 proteins was analyzed from A549 cells; (C) Cells were immunostained with p62 antibody (red) and observed in fluorescent view; (D) Western blot for Ac-cas3 and Ac-cas8 expression levels was conducted. A549 cells were pre-incubated with ATG5siRNA or negative control siRNA for 24 h, and then exposed to indicated glipizide doses for 12 h with or without TRAIL protein for an additional 1 h. β-actin was used as the loading control. Gli: Glipizide; TRAIL: Tumor necrosis factor (TNF)-related apoptosis-inducing ligand; Ac-cas3: Activated caspase 3; Ac-cas8: Activated caspase 8; siATG5: ATG5 small interfering RNA; NC: Negative control.