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. 2017 Oct 26;8(59):100187–100195. doi: 10.18632/oncotarget.22124

Figure 5. Knockdown of JMJD3 inhibits calcitriol-induced p16INK4A upregulation and cell senescence.

Figure 5

(A) 786-O cells were transfected with JMJD siRNA or control (Ctl) siRNA, and the knockdown effects were determined with qRT-PCR (up) and western blotting (down). (B) 786-O cells transfected with Ctl siRNA or JMJD3 siRNA were treated with vehicle (Veh) or 100nM calcitriol (Cal) for 48h, and cell senescence was determined by SA-β-gal staining. (C) Quantitative results of cell senescence. (D) The expression of p16INK4A in 786-O cells first transfected with Ctl siRNA or JMJD3 siRNA, and then treated with Veh or 100nM Cal for 48h. Scale bar, 100μm. Magnification, 200×. *, P < 0.05.