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. 2017 Oct 31;8(59):100269–100279. doi: 10.18632/oncotarget.22212

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Copyright: © 2017 He et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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(A) A DP from mouse whisker is expanded during culture. (B) DP cells grow in a spindle shape after 1 passage culture. (C) Primary epithelial cell culture from mouse hair follicle. (D) Epithelial cells maintain their morphology after 1 passage culture. (E) Immunostaining shows positive a-SMA expression in cultured DP cells. (F) DP cells show positive alkaline phosphatase staining after 1 passage culture. (G) Statistical analysis reveals that more than 80% P1 cells are aSMA positive; (H) Statistical analysis reveals that about 80% P1 cells are alkaline phosphatase (AP) positive; P1, subculture for 1 passage. N=9.