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. 2017 Nov 1;8(59):100358–100370. doi: 10.18632/oncotarget.22229

Figure 2. The establishment of stable KLF2 overexpressing and knockdown GC cell lines.

Figure 2

(A) Protein expression of KLF2 in ten human GC cell lines and one normal gastric cell line via Western blot. MKN-45 and BGC-823 express relatively low levels KLF2 whereas MGC-803 cell line expresses relatively high levels of KLF2. (B and C) MKN-45 and BGC-823 cancer cell lines were infected with Vector and KLF2-overexpressing lentivirus. The level of KLF2 in MKN-45 and BGC-823 cell lines was verified by Western blot. (D and E) MGC-803 cell line was infected with scambled and KLF2 targeting shRNA lentivirus. Western blot to verify the level of KLF2 in the MGC-803 knockdown cell line.