Figure 9. Effect of Sirtinol on H₂S-meliorated hippocampal apoptosis in CRS-treated rats Rats were cotreated with NaHS (100 μmol/kg/d, i.p.) and CRS (6 h/d) for 4 w and injected with Sirtinol (10 nmol/d×1w, i.c.v.) at the last week simultaneously.

(A) The level of apoptosis was detected by tunel staining (Left, magnification x400). (B and C) The apoptotic-associated proteins Bax (B) and Bcl-2 (C) were measured by western blotting. Values are the mean ± S.E.M. (n=3). ^*P < 0.05, ^**P < 0.01, vs control group; ^#P < 0.05, ^##P < 0.01, vs CRS-treated alone group; ^&P < 0.05, ^&&P < 0.01, vs cotreated with NaHS and CRS (100 μmol/kg/d, i.p.) treated group.