Table 1. qPCR primer sequences.
| Gene name | Forward (5’ to 3’) | Reverse (5’ to 3’) | Product size (bp) | Accession number |
|---|---|---|---|---|
| Prdm16 | CAGCACGGTGAAGCCATTC | GCGTGCATCCGCTTGTG | 87 | XM_006539178.3 |
| Ucp1 | ACTGCCACACCTCCAGTCATT | CTTTGCCTCACTCAGGATTGG | 123 | NM_009463.3 |
| Pgc1a | CCTCACACCAAACCCACAGA | CCTCATGCGGTCACTGTCG | 272 | XM_006503779.3 |
| Cidea | ATCACAACTGGCCTGGTTACG | TACTACCCGGTGTCCATTTCT | 136 | NM_007702.2 |
| Elovl3 | GATGGTTCTGGGCACCATCTT | CGTTGTTGTGTGGCATCCTT | 73 | XM_006526624.3 |
| Cox7a1 | CAGCGTCATGGTCAGTCTGT | AGAAAACCGTGTGGCAGAGA | 112 | NM_009944.3 |
| Aldh1a1 | CCTTGCATTGTGTTTGCAGATG | GCTCGCTCAACACTCCTTTTC | 158 | NM_013467.3 |
| IL-1β | TCGCTCAGGGTCACAAGAAA | CATCAGAGGCAAGGAGGAAAAC | 73 | XM_006498795.3 |
| IL-18 | ATGCTTTCTGGACTCCTGCCTGCT | GGCGGCTTTCTTTGTCCTGATGCT | 89 | XM_006510028.3 |
| IL-6 | GAGGATACCACTCCCAACAGACC | AAGTGCATCATCGTTGTTCATACA | 141 | NM_001314054.1 |
| Pax7 | TTGGGGAACACTCCGCTGTGC | CAGGGCTTGGGAAGGGTTGGC | 115 | XM_006538631.1 |
| Myf5 | AAACTCCGGGAGCTCCGCCT | GGCAGCCGTCCGTCATGTCC | 125 | XM_006513319.2 |
| MyoD | TCTGGAGCCCTCCTGGCACC | CGGGAAGGGGGAGAGTGGGG | 100 | NM_010866.2 |
| MyoG | GAGATCCTGCGCAGCGCCAT | CCCCGCCTCTGTAGCGGAGA | 97 | NM_031189.2 |
| 18s rRNA | TTGTACACACCGCCCGTCGC | CTTCTCAGCGCTCCGCCAGG | 102 | NR_003278.3 |