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EAC cell lines OE19 (Panel A and B), OE33 (Panel C and D), and FLO1 (Panel E and F) were utilized to evaluate the effects of abemaciclib on apoptosis through flow cytometry analysis using Annexin-V and Calcein to stratify early and late apoptotic effects. With treatment, total apoptosis increased across all three cell lines by 123.9%, 103.7%, 145.5%, respectively. Early apoptosis also increased by 172.7%, 229.7%, 108.7%, respectively.
