Table 4. Immunohistochemical analysis of the eligible studies.
| Study | Tissue section | Primary antibody | Dilution | Compartment | Definition of ki-67 index | % IHC cut-off | % ki-67 positive | Interpretation |
|---|---|---|---|---|---|---|---|---|
| Asakura [20] | All specimens | NA | 1:200 | Nuclei | Yes | 5.35 | 50 | NA |
| Lee [21] | All specimens | NA | NA | Nuclei | Yes | 16 | 50 | Blind |
| Pfister [22] | All specimens | Monoclonal | 1:50 | Nuclei | No | 10 | 70 | Blind |
| Tomobe [23] | All specimens | NA | 1:200 | Nuclei | Yes | 15.5 | 50 | NA |
| Wu [24] | All specimens | NA | 1:100 | Nuclei | Yes | 10.9 | 50 | Blind |
| Blanchet [25] | All specimens | Monoclonal | NA | NA | Yes | 13 | 18.5 | Blind |
| Kamai [26] | All specimens | Monoclonal | NA | Nuclei | Yes | 30 | 18.6 | NA |
| Kilicli-Camur [27] | All specimens | Monoclonal | 1:30 | Nuclei | Yes | 25 | NA | NA |
| Sgambato [28] | All specimens | Monoclonal | 1:100 | Nuclei | Yes | 10 | 65.6 | Blind |
| Yan [29] | All specimens | NA | NA | Nuclei | No | 25 | 34.2 | NA |
| Dybowski [30] | All specimens | Monoclonal | 1:50 | Nuclei | No | 30 | 50 | Blind |
| Santos [31] | All specimens | NA | 1:50 | Nuclei | Yes | 18 | 50 | NA |
| Su [32] | All specimens | NA | 1:50 | Nuclei | Yes | 18 | 50 | NA |
| Mhawech [33] | TM (1.6 mm core) |
NA | 1:50 | Nuclei | Yes | NA | 50 | Blind |
| Krüger [34] | TM (2 × 2 mm) | Monoclonal | 1:20 | Nuclei | Yes | Continuous | - | Blind |
| Theodoropoulos [35] | All specimens | NA | Prediluted | Nuclei | Yes | 8.6 | 50 | Blind |
| Gonzalez-Campora [36] | All specimens | Monoclonal | 1:20 | Nuclei | Yes | 10 | 18.4 | NA |
| Quintero [37] | All specimens | Monoclonal | Prediluted | Nuclei | Yes | 13 | 10.4 | NA |
| Yin [38] | All specimens | Monoclonal | 1:100 | Nuclei | Yes | 20 | 24.8 | NA |
| Maeng [39] | All specimens | NA | 1:80 | Nuclei | Yes | 25 | 36.4 | NA |
| Miyake [40] | All specimens | Monoclonal | Prediluted | Nuclei | Yes | 25 | 40.4 | Blind |
| Seo [41] | All specimens | Monoclonal | 1:50 | Nuclei | Yes | 25 | 36.4 | NA |
| van Rhijn [10] | All specimens | NA | NA | NA | NA | 25 | NA | Blind |
| Behnsawy [42] | All specimens | Monoclonal | 1:200 | Nuclei | Yes | 5 | 28.6 | Blind |
| Wosnitzer [43] | All specimens | Monoclonal | NA | NA | Yes | 10 | 50 | Blind |
| Acikalin [6] | All specimens | Monoclonal | 1:50 | Nuclei | Yes | 10 | 69.1 | Blind |
| Chen [11] | All specimens | Monoclonal | 1:50 | Nuclei | Yes | 25 | 47.2 | NA |
| Ogata [44] | All specimens | Monoclonal | 1:100 | NA | No | 20 | 58.1 | NA |
| Oderda [45] | All specimens | Monoclonal | 1:10 | Nuclei | Yes | 20 | NA | NA |
| Okazoe [46] | All specimens | Monoclonal | 1:100 | Nuclei | Yes | 18 | 29.6 | Blind |
| Park [47] | TM (1 mm core) |
Monoclonal | 1:200 | Nuclei | Yes | 10.4 | 40 | Blind |
| Ruan [48] | All specimens | Polyclonal | 1:50 | Nuclei | Yes | 10 | 55.6 | Blind |
| Ben Abdelkrim [14] | All specimens | NA | 1:50 | Nuclei | Yes | 10 | 38 | Blind |
| Bertz [18] | All specimens | Monoclonal | 1:50 | Nuclei | Yes | 15 | 64.4 | NA |
| Ding [15] | All specimens | Monoclonal | 1:100 | Nuclei | No | 25 | 32.5 | NA |
| Mangrud [49] | All specimens | NA | NA | NA | Yes | 39 | 25 | NA |
| Pan [50] | TM (2 mm core) |
NA | 1:100 | Nuclei | Yes | 20/80 | NA | Blind |
| Özyalvaçli [16] | All specimens | Monoclonal | NA | Nuclei | Yes | 10 | 27.8 | Blind |
| Poyet [17] | TM (1 mm core) |
NA | 1:50 | NA | Yes | 10 | 38.4 | NA |
IHC: immunohistochemistry, NA: not available, TM: tissue microarray.