Table 4. Comparison of EGFR T790M detection platforms in tissue.

S.No	Method	Sample	EGFR T790M Detection rate %		Study group
			Treatment Naive/Pre-treatment	Post-TKI
1	Scorpion ARMS	Tissue N = 29	0	48.3	Chen HJ et al. [84]
2	Direct sequencing	Tissue N = 14	0	50	Kosaka et al. [75]
3	ARMS	Tissue N = 10	-	0	Zhang et al. [85]
	ddPCR		-	50
4	Standard HRM	Tissue N = 146	0	-	Hashida et al. [107]
	MEC-HRM		13	-
5	SABER	Tissue N = 28	7	-	Sakai et al.[120]
6	Ion Torrent PGM NGS	Tissue N = 15	-	60	Masago et al. [94]
7	ddPCR	Tissue N = 12	83.3	-	Isobe K et al. [92]
8	MALDI-TOF MS	Tissue N = 54	7.1	-	Su K.Y et al. [97]
	NGS		14.3	-
9	PNA-clamping PCR	Tissue N = 50	-	68	Costa C et al. [110]
10	ddPCR	Tissue N = 78	6.4	-	Xu et al. [93]
11	ACB-ARMS PCR	Tissue N = 27	22.2	-	Zhao J et al. [83]
12	PNA-clamping PCR	Tissue N = 147	8.2	-	Oh et al. [76]
	Direct sequencing		0	-
13	ddPCR	Tissue N = 373	79.9	-	Watanabe M et al.[105]
14	Direct sequencing	Tissue + other clinical samples N = 280	0.3	1.05	Inukai M et al. [77]
	Mutant-enriched PCR		3.5	3.1
15	TaqMan PCR	Tissue N = 129	35	-	Rosell R et al. [122]
16	SARMS	Tissue N = 38	0	-	Fujita Y et al. [86]
	Colony hybridisation		79	-
17	Direct sequencing	Tissue N = 98	2	-	Sequist LV et al. [71]
18	Direct sequencing	Tissue+other clinical samples N = 1261	0.5	-	Wu JY et al. [79]
19	NGS (Miseq/Hiseq2000/Hiseq2500)	Tissue N = 209	0.48	-	Hagemann IS et al.[108]
20	LNA-PCR sequencing	Tissue N = 155	-	62	Yu HA et al. [111]
21	Direct sequencing	Tissue+other clinical samples N = 69	-	49	Arcila ME et al. [80]
	RFLP-PCR	Tissue+other clinical samples N = 45	-	53
	LNA-PCR sequencing	Tissue+other clinical samples N = 64	-	70
22	TaqMan PCR	Tissue+other clinical samples N = 15	-	40	Molina-Vila MA et al. [123]
23	AMRS	Tissue N = 609	0.8	-	Mok TS et al. [87]
24	Direct sequencing	Tissue N = 74	-	1.35	Soh J et al. [81]
25	Cobas(Roche)/ARMS (Qiagen)	Tissue N = 148	-	53	Sequist LV et al. [101]
26	Cobas (Roche)	Tissue N = 222	-	62	Janne PA et al. [21]
27	ARMS	Tissue N = 134	6.8	28.4	Yu J et al. [124]
28	NGS (MiSeq)	Tissue = 15	-	73.3	Paweletz et al. [95]
29	NGS (AmpliSeq cancer hotspot panel v2)	Tissue N = 43	-	79	Belchis DA et al. [96]

‘-‘: not reported