Figure 3. 64B downregulates mTORC1 signaling and disrupts HIF-1 transactivation in tumor cells.

Tumor cells were cultured in normoglycemic or hyperglycemic DMEM under normoxia (21% O₂) or hypoxia (1% O₂), and were treated with 64B (5 μM) for 24 h. A,B, Western blot analysis showing the effect of 64B on the levels of indicated proteins under normoxia (A) and hypoxia (B). C, qRT-PCR measuring the expression of HIF-1α, Glut-1 and VEGF mRNAs in 64B-treated tumor cells under hypoxia. All data show representative results obtained from three independent experiments, and the results are reported as the mean ± SD (n = 3). *, p < 0.05. **, p < 0.01.