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. 2017 Jul 26;8(59):99414–99428. doi: 10.18632/oncotarget.19562

Figure 3. Overexpression of AQP1 promoted proliferation and invasion abilities of LN229 cells.

Figure 3

(A) In parental LN229 cells, AQP1 expression was too low to be detected by western blot. Mouse kidney tissue was used as a positive control. (B) Establishment of stable AQP1-overexpressing cells through lentiviral transfection of GFP labeled AQP1-expressing vector (upper part) and 3×Flag labeled AQP1-expressing vector (lower part). (C) Exogenous AQP1 expression was observed in AQP1/LN229 (upper part) and 3×Flag-AQP1/LN229 cells (lower part) by GFP fluorescence or anti-AQP1 antibody, respectively (200×). (D) Proliferation ability was detected by BrdU incorporation analysis in AQP1/LN229 and control cells (200×). (E) Proliferation assay was performed by MTT assays in AQP1/LN229 and control cells. (F) Colony-forming ability was examined in AQP1/LN229 and control cells (200×). (G) Invasion assays were performed in AQP1/LN229 and control cells (200×). All experiments were performed 3 times independently (Student’s t-test, *P < 0.05, **P < 0.01).