Skip to main content
. 2017 Jul 26;8(59):99414–99428. doi: 10.18632/oncotarget.19562

Figure 5. Relationship between AQP1 and β-catenin in astrocytoma.

Figure 5

(A) Expression of AQP1 was positively correlated with β-catenin expression. The expression of AQP1 and β-catenin were detected using serial paraffin sections by immunohistochemistry analysis. (B) The expression of AQP1 and β-catenin were detected by western blot using tumor tissues from astrocytoma patients (7 cases). (C) Co-localization of AQP1 and β-catenin in AQP1/LN229 and 3×Flag-AQP1/LN229 cells, respectively (200×). (D) Co-immunoprecipitation results of AQP1 and β-catenin in astrocytoma tissues. (E) Co-immunoprecipitation results of AQP1 and β-catenin in 3×Flag-AQP1/LN229 cells. (F) β-catenin was upregulated in AQP1/LN229 cells compared with control. (G) Knocking down β-catenin expression in LN229 and AQP1/LN229 cells (#1, #2 and #3). The vector/LN229 cell line was used as a control group. (H) Proliferation experiments by MTT assays. (I) Migration experiments results (200×). (J) Invasion experiments results (200×). All experiments were performed 3 times independently (Student’s t-test, *P < 0.05, **P < 0.01).