Table 1. PCR primer sequences and amplification parameters.
Gene | Primer sequence (5’-3’) | Product size (bp) | Tm (°C) |
---|---|---|---|
β-actin | GGTCATCACTATTGGCAACGAG | 142 | 57 |
GAGGTCTTTACGGATGTCAACG | |||
PC1 | AGTTGGAGGCATAAGAATGCTG | 159 | 59 |
GCCTTCTGGGCTAGTCTGC | |||
PG | ACTTTGTGGCTGGATTGCTT | 146 | 58 |
GTGGCGTTTGTCTTCATTCA | |||
CD18 | CTGACCCACCTGACTGACCT | 109 | 58 |
TGACCGTTGTCGTAGCACTC | |||
aP2 | AAGGTGAAGAGCATCATAACCCT | 133 | 61 |
TCACGCCTTTCATAACACATTCC | |||
PPARγ | GGAAGACCACTCGCATTCCTT | 121 | 58 |
GTAATCAGCAACCATTGGGTCA | |||
CD36 | ATGGGCTGTGATCGGAACTG | 110 | 57 |
GTCTTCCCAATAAGCATGTCTCC |