Figure 4. The effect of ICA on protein expression levels of MAPK signaling molecules in B16 cells.

Cells were exposed to ICA at 25, 50 and 100 μM for 24 h. (A) The protein level of Erk1/2, p-Erk1/2, p38, p-p38, JNK and p-JNK were examined by Western blot. (B) Western blot analysis of Erk1/2, p-Erk1/2, p38, p-p38, JNK and p-JNK protein level in ICA-treated B16 cells. All data are the mean ± S.D. of three independent experiments. ^*P < 0.05, ^**P < 0.01 compared with control group.