Figure 2.

(A) RUNX1 knocking down efficiency was measured by RT-qPCR assay in two cells (siRUNX1-1 and siRUNX1-2 are different siRNA fragments). ^** P < 0.01. (B) Overexpression of RUNX1. Cells were transfected with pcDNA3.1-RUNX1 or empty vector pcDNA3.1. Overexpression of RUNX1 was confirmed by RT-qPCR (Left) and Western blotting (Right) in two cells, respectively. ^**P < 0.01, t test. (C, D) Wound healing assay. PANC-1 cell was transfected with Negative Control (NC) and si-RUNX1. Optical images of wounded cell monolayers were taken after 0 h, 24 h, 48 h, and 72h. The remaining distance was calculated as a percentage of the initial wound area. ^*P<0.05 or ^**P<0.01. (E, F) Two pancreatic cell lines transfected with NC, si-RUNX1 and pcDNA3.1-RUNX1 cells were collected and induced to invade through Matrigel-coated transwell membranes. The numbers of migrated cells were counted. ^** P < 0.01.