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. 2017 Aug 24;8(59):99624–99636. doi: 10.18632/oncotarget.20454

Figure 1. CDC-EVs negatively impact the aggressiveness of human HT1080 fibrosarcoma cells in vitro.

Figure 1

(A) Priming of HT1080 cells with CDC-EVs was associated with a favorably balanced modulation of cancer progression-related proteins compared to culture in serum-free media (SF) alone (lilac bars). Only significantly modulated (p<0.05) proteins are shown. Black bars represent the protein expression levels in the SF cells. (B) Representative wells and a decreased invasion capacity of HT1080 after priming the cells with CDC-EVs vs SF. (C) Representative wells and a decreased adhesion capacity of HT1080 after priming the cells with CDC-EVs vs SF. (D) Priming of HT1080 cells with CDC-EVs was associated with a down-regulation of many “cancer drug target” genes. Only significant (p<0.05) and more than two-fold modulated genes are shown. (E) Telomerase activity in extracts of HT1080 cells was determined following telomeric repeat amplification protocol in SF and CDC-EV primed cells, showing a marked decrease in the later group of cells. *p<0.05. Bar graphs represent mean (±SEM). Minimum number of replicates per experiment was 3.