Figure 8. Effect of silibinin on IL-1β-induced PI3K/AKT phosphorylation in human OA Chondrocytes.

Chondrocytes were pretreated with silibinin (1, 10, 50 μM) for 2h, followed by stimulation with or without IL-1β (10 ng/ml) for 1 h. The protein expression levels of PI3K, p-PI3K, AKT and p-AKT were determined by Western blot and quantification analysis (A-C). The values are mean ± SD. ^#p < 0.05 compared with control group, ^*p < 0.05, ^**p< 0.01 compared with IL-1β group. n=6.