Figure 5. Pyroptosis assay of monocytes infected with ALV-J strain SCAU-HN06.

(A) Monocytes isolated from SPF chickens were infected with SCAU-HN06 (10^4.5 TCID₅₀/0.1 mL). Uninfected monocytes represented natural control (NC). Cells were stained by propidium iodide (PI) (red indicated pyroptotic cells) and Hoechst 33342 (blue indicated all cells) at 24 hpi. Images were captured and merged using fluorescence microscopy (300 ×). Three independent experiments were performed (each in triplicate), and representative data from one experiment was shown. (B) Pyroptosis of five random images containing all cells were calculated. ^***P < 0.001.