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. 2017 Oct 7;8(59):99950–99965. doi: 10.18632/oncotarget.21556

Figure 5. 3C23K mAb reduces macrophages induced-T cells immunosuppression.

Figure 5

(A) Measure of PBT proliferation co-cultured with MDM2 macrophages targeting COV434-AMHRII tumor cells. MDM2 were challenged with COV434-AMHRII opsonized with either the irrelevant mAb R565 (isotype Ctrl), the anti-AMHRII FcKO or the anti-AMHRII 3C23K for 4 days prior the co-culture for an additional 4 days with anti-CD3/CD28 pre-activated PBT. Data represent the Division Index (i.e. the average number of cell divisions that a cell in the original population has undergone) of pre-activated CD8+ T cells +/- Standard Deviation. (Data are representative of three independent experiments. P-values * < 0.05). (B) Measure of PBT proliferation co-cultured with MDM2 macrophages targeting mAb treated polystyrene beads. MDM2 were challenged with polystyrene beads non coated as control or coated with either the anti-AMHRII FcKO or the anti-AMHRII 3C23K for 24 hours prior the co-culture for an additional 4 days with pre-activated cell trace violet loaded PBT. Data represents the Division Index (i.e. the average number of cell divisions that a cell in the original population has undergone) of pre-activated CD8+ T cells +/- Standard Deviation. (Data are representative of three independent experiments. P-values ** < 0.01). “n.a” refers to non-activated and a. to activated T cells.