Figure 3.

Expression of PTEN-L and lclPTENL

(A) U87MG cells engineered for inducible expression of PTEN-L or lclPTEN-L were treated with 1 μM doxycycline for 48 hr. U87MG cells were grown in media containing 0.5% FBS to reduce interference of the BSA band in western blots of conditioned media. Total cell lysates and conditioned media were collected and analyzed by western blot. The top panel shows the blot probed with PTEN antibody. The bottom panel shows that same blot stained with amido black to show equal loading of total protein in each lane. (B) U87MG cells expressing lclPTEN-L were treated with doxycycline for the indicated period of time. Conditioned media were then analyzed by western blotting with antibody to PTEN. (C) U87MG cells expressing lclPTEN-L were treated without or with doxycycline for 24 hr in the presence of the indicated concentrations of brefeldin A. Conditioned media were then analyzed by western blotting using antibody to PTEN.